MT-CYB

Today, we want to delve into the fascinating world of MT-CYB. Since time immemorial, MT-CYB has aroused the interest and curiosity of millions of people around the world. Whether due to its impact on society, its historical relevance or its influence in the cultural sphere, MT-CYB has left an indelible mark on humanity. Throughout this article, we will explore the various aspects that make MT-CYB a topic of universal interest, addressing both its past, its present and its future. Get ready to immerse yourself in an exciting journey through history, current events and the possibilities that MT-CYB has in store for us.

CYTB
Identifiers
AliasesCYTB, MTCYB, MT-CYB, cytochrome b
External IDsOMIM: 516020; MGI: 102501; HomoloGene: 5013; GeneCards: CYTB; OMA:CYTB - orthologs
Orthologs
SpeciesHumanMouse
Entrez

4519

17711

Ensembl

ENSG00000198727

ENSMUSG00000064370

UniProt

P00156

P00158

RefSeq (mRNA)

n/a

n/a

RefSeq (protein)

n/a

NP_904340

Location (UCSC)Chr M: 0.01 – 0.02 MbChr M: 0.01 – 0.02 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
Location of the MT-CYB gene in the human mitochondrial genome (coral box).

Cytochrome b is a protein that in humans is encoded by the MT-CYB gene.[5] Its gene product is a subunit of the respiratory chain protein ubiquinol–cytochrome c reductase (UQCR, complex III or cytochrome bc1 complex), which consists of the products of one mitochondrially encoded gene, MT-CYB (mitochondrial cytochrome b), and ten nuclear genes—UQCRC1, UQCRC2, CYC1, UQCRFS1 (Rieske protein), UQCRB, "11kDa protein", UQCRH (cyt c1 Hinge protein), Rieske protein presequence, "cyt c1 associated protein", and Rieske-associated protein.

Structure

The MT-CYB gene is located on the p arm of mitochondrial DNA in position 12 and spans 1,140 base pairs.[5] The gene produces a 42.7 kDa protein named cytochrome b composed of 380 amino acids.[6][7] Cytochrome b is an integral membrane protein with hydrophobic properties. The catalytic core of the enzyme is composed of eight transmembrane helices, the iron-sulfur protein, and cytochrome c1.[8] Cytochrome b is a fundamental component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c.[9] The structure of the complex is a symmetric homodimer. It is composed of eleven structural subunits, including one mitochondrial genome encoded cytochrome b and ten other nucleus encoded subunits. These subunits include three respiratory subunits (MT-CYB, CYC1 and UQCRFS1), two core proteins (UQCRC1 and UQCRC2) and six low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). The total molecular mass of the complex is about 450 kDa.[10][9]

Function

The mitochondrial cytochrome b is fundamental for the assembly and function of Complex III of the mitochondrial respiratory chain.[11] Complex III is responsible for the catalysis of electron transfer from coenzyme Q to cytochrome c in the mitochondrial respiratory chain by translocating protons concomitantly across the inner membrane of the mitochondria.[12][9] The transfer of electrons then contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.[9]

Clinical significance

Mutations in MT-CYB can result in mitochondrial deficiencies and associated disorders. It is majorly associated with a complex III deficiency, a deficiency in an enzyme complex which catalyzes electron transfer from coenzyme Q to cytochrome c in the mitochondrial respiratory chain. A complex III deficiency can result in a highly variable phenotype depending on which tissues are affected.[9] Most frequent clinical manifestations include progressive exercise intolerance and cardiomyopathy. Occasional multisystem disorders accompanied by exercise intolerance may arise as well, in forms of deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, and epilepsy.[9] Other phenotypes include mitochondrial encephalomyopathy, mitochondrial myopathy, Leber hereditary optic neuropathy, muscle weakness, myoglobinuria, blood acidosis, renal tubulopathy, and more.[9][10] Complex III deficiency is known to be rare among mitochondrial diseases and may follow a maternal or mendelian mode of inheritance due to its duality of genetic origin.[8]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000198727 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000064370 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b "Entrez Gene: CYTB cytochrome b".
  6. ^ Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P (Oct 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043–53. doi:10.1161/CIRCRESAHA.113.301151. PMC 4076475. PMID 23965338.
  7. ^ "cytochrome b". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).
  8. ^ a b Fragaki K, Procaccio V, Bannwarth S, Serre V, O'Hearn S, Potluri P, Augé G, Casagrande F, Caruba C, Lambert JC, Paquis-Flucklinger V (September 2009). "A neonatal polyvisceral failure linked to a de novo homoplasmic mutation in the mitochondrially encoded cytochrome b gene". Mitochondrion. 9 (5): 346–52. doi:10.1016/j.mito.2009.06.002. PMID 19563916.
  9. ^ a b c d e f g "UniProtKB - P00156 (CYB_HUMAN)". The UniProt Consortium.
  10. ^ a b Gil Borlado MC, Moreno Lastres D, Gonzalez Hoyuela M, Moran M, Blazquez A, Pello R, Marin Buera L, Gabaldon T, Garcia Peñas JJ, Martín MA, Arenas J, Ugalde C (September 2010). "Impact of the mitochondrial genetic background in complex III deficiency". PLOS ONE. 5 (9): e12801. Bibcode:2010PLoSO...512801G. CiteSeerX 10.1.1.350.7243. doi:10.1371/journal.pone.0012801. PMC 2941448. PMID 20862300.
  11. ^ Massie R, Wong LJ, Milone M (July 2010). "Exercise intolerance due to cytochrome b mutation". Muscle & Nerve. 42 (1): 136–40. doi:10.1002/mus.21649. PMID 20544923. S2CID 23759055.
  12. ^ Baum H, Rieske JS, Silman HI, Lipton SH (March 1967). "On the mechanism of electron transfer in complex iii of the electron transfer chain". Proceedings of the National Academy of Sciences of the United States of America. 57 (3): 798–805. Bibcode:1967PNAS...57..798B. doi:10.1073/pnas.57.3.798. PMC 335578. PMID 16591533.

Further reading