Cragie tube
In today's world, Cragie tube has become a topic of constant interest and debate. Whether due to its impact on society, its historical relevance or its influence in different areas, Cragie tube has captured the attention of academics, specialists and the general public. Over the years, the study and analysis of Cragie tube has been deepened, seeking to understand its causes, consequences and possible solutions. This article seeks to offer a general and complete overview of Cragie tube, addressing its different aspects from an objective and analytical perspective. From its origin to its implication today, the different facets of Cragie tube will be explored, in order to provide the reader with a deeper and more precise understanding of this topic of universal relevance.
The Cragie tube or Craigie tube is a method used in microbiology[1][2] for determining bacterial motility.
Technique
A hollow tube with some culture medium is placed in semi-solid agar inside a bottle. A sample of the bacterium to be tested is inoculated into the medium in the hollow tube and the setup is incubated at 37 °C overnight.
Observation
On examining the areas where bacterial growth has occurred there are several observations to be made:
- the colonies of the non-motile bacteria remain confined within the tube at the site of inoculation
- the motile bacteria swim out from the bottom of the tube and colonize the surrounding medium as well
Confirmation may be obtained by subculture and retesting.
References
- ^ Textbook of Microbiology by Prof. C P Baveja, ISBN 81-7855-266-3
- ^ Textbook of Microbiology by Ananthanarayan and Panikar, ISBN 81-250-2808-0
See also
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